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OBJECTIVE@#To investigate the effects of 650 nm laser irradiation on cell oxygen consumption rate in CC myoblasts following different doses.@*METHODS@#CC cells were irradiated with 650 nm laser(λ=650 nm, p=5 mW) with energy densities of 0, 0.4, and 0.8 J/cm. Cell oxidative function was measured by oxygen consumption rate kit. Protein expression of myogenic determination factor (MyoD), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), mammalian target of rapamycin (mTOR) and its phosphorylation were detected by Western blot.@*RESULTS@#Compared to the control group, the expression levels of MyoD, PGC-1α protein were increased and cell oxygen consumption rate was promoted in the low dose group(<0.05). MyoD and PGC-1α protein expressions were also increased(<0.05), the ratio of mTOR and its phosphorylationwere decreased significantly in the high dose group(<0.05).@*CONCLUSIONS@#650 nm laser irradiation that dose is 0.4 J/cm enhances cell oxidative function, it related to that proper dose laser irradiation promoted the expression of PGC-1a protein.
Subject(s)
Animals , Mice , Cell Line , Myoblasts , Oxidation-Reduction , Oxygen Consumption , Transcription FactorsABSTRACT
Objective:To investigate the effects of two drugs on the asphyxia oxygen concentration and the oxygen consumption rate of zebrafish,and explore the possibility of zebrafish as a drug model and the feasibility of oxygen microelectrode used for drug screening. Methods:The zebrafish were treated with the drugs for 5 days,and transferred into the asphyxia chambers. The solute oxy-gen concentration was assessed continuously until the asphyxia condition. The asphyxia oxygen concentration,asphyxia time and mass specific oxygen consumption rate were determined. Results:The results showed that the asphyxia time,asphyxia concentration and ox-ygen consumption rate of the zebrafish were not significantly changed by deproteinized calfblood extractives injection(P>0.05),sug-gesting no reduction in hypoxia tolerance ability. The asphyxia time and oxygen consumption rate of the zebrafish were not significantly changed,either (P>0.05),however,the asphyxia concentration was increased significantly by cervus and cucumis polypeptide injec-tion (P<0.05),suggesting the reduction in hypoxia tolerance ability. Conclusion:It is suggested that the hypoxia tolerance ability of zebrafish can be significantly decreased by the solution of cervus and cucumis polypeptide. The results also suggest that zebrafish can be used as an experimental animal in medicine studies,and the oxygen microelectrode method can be used in drug screening.
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The biological characteristics, oxygen consumption, oxygen consumption rate, and activities of amylase, lipase and protease of Whitmania pigra at different temperature were studied by using direct observational method, the still water method and 3, 5-dinitrosalicylic acid colorimetry, right-nitrophenyl palmitate ester（ρ-NPP）colorimetry and folin-phenol method.The results revealed that with decreasing water temperature, the daily activity and the daily feeding ration were decreased. As the temperature was lowered to 4 ℃, the head and tail of Wh.pigra curved, showing a crescent-shape without feeding and daily activity. Oxygen consumption, oxygen consumption rate and digestive enzyme activities reduced along with temperature drops. The downward trend slowed below 10 ℃, began to stabilize below 4 ℃ and doesn't change with the decrease of temperature since then. During the 40 days treatment at 4 ℃, the changes of amylase were not significant, the lipase and protease activity decreased at the 20th day, and the lipase showed an slightly increase after the decrease and finally remained at a low level.In conclusions, the pivotal temperature of hibernation of Wh.pigra is 4 ℃ and the crescent shape can be considered as a symbol of hibernation.
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The oxygen consumption, oxygen consumption rate and suffocation point of different quality Whitmania pigra and Bellamya purificata were determined by hydrostatic breathing room method. The effects of feeding modes on growth of W.pigra were determined by biomass. The results showed that the oxygen consumption correlated positively with the weight of W.pigra and B. purificata（P<0.05）, suffocation point increased with the increases of the weight（P<0.05）.Oxygen consumption correlated negatively with the weight of W. pigra, the oxygen consumption rate of B.purificata first increased and then decreased with the increasing of the weight. Feeding modes had no significant effects on the finial weight, SGR, WGR, death rates of W. pigra. Feeding modes had significant effects on eating ratio. It suggested that the optimum feeding frequency of W. pigra was once every three days. Scientific and reasonable feeding amount of B. purificata should be calculated based on oxygen consumption and suffocation point of W.pigra and B.purificata at every period. Meanwhile, stocking density, water area and water exchanging frequency should be taken into consideration.
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In this article,the authors reported a modified method of determining Oxygen consump- tion for the mouse hypoxia experiment in order that the students could observe the dynamic change of oxygen consumption rate(OCR)and respiratory rate(RR)in the whole process of acute progressive hypoxic hypoxia on mice.The results showed that to reach the maximal OCR and RR,especially the latter,required more time as the survival time was prolonged. In a closed set of the same size,the heavier the mouse weighed,the shorter it survived,but there was an individual difference.